update 17/01/2021, online 20/01/2001
the ATP synthase project:
Monitoring
inter-subunit
rotation in a
biochemical
nanomotor
during
catalysis
- now hold
in solution
by a fast
ABELtrap.
DIY progress @ 2020 :
getting a single FRET-labeled FoF1-ATP synthase into an
Anti-Brownian electrokinetic trap (ABELtrap) and recording
fast subunit rotation during ATP hydrolysis at 100µM [ATP].
Labeling positions yield two distinct FRET levels ‘L’ and ‘H’.
Single-molecule catalysis results in fluctuating FRET levels.
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