Michael Börsch: Studies. Postdoc. 'Wissenschaftlicher Assistent'. Research Scientist

 @ 1983/84 Chemistry (Diploma) at the Albert-Ludwigs-Universität Freiburg (‘University of Freiburg’), Germany
     main topics: Physical Chemistry, Polymer Science

     03/06/1991 Diploma, with Prof. Dr. H.W. Zimmermann, Institute of Physical Chemistry, University of Freiburg,
     19/12/1996 Dr. rer. nat., with Prof. Dr. H.W. Zimmermann

 @ 01/01/1997 Postdoc and
       01/05/1999  'Wissenschafticher Assistent' with Prof. Dr. P. Gräber  at the Institute of Physical Chemistry,
     Albert-Ludwigs-Universität Freiburg:

       Setup of a confocal two-channel fluorescence-correlation spectrometer for single-molecule detection and timeresolved
       single-molecule-FRET with ATP synthase

 @ 01/04/2002 Research Scientist with Prof. Dr. Jörg Wrachtrup  at the 3rd Institute of Physics, University of Stuttgart.
     (permanent position):

       Single-molecule FRET imaging of liposome-immobilized ATP synthases and setup of a biochemistry laboratory
 

Scientific Interests

1. Fluorescence spectroscopy of the conformational changes of single H⁺-ATP synthases from E. coli by single-molecule FRET
with Prof. Dr. P. Gräber (University of Freiburg, Germany), Prof. Dr. S. Dunn (University of Western Ontario, London, Ontario, Canada)

 2. Immobilization methods of single H⁺-ATP synthases in liposomes and lipid bilayers in glass nanopores, FRET imaging,
with Prof. Dr. J. Wrachtrup (University of Stuttgart) in the network of competence ‘functional nanodevices' by the Landesstiftung Baden-Württemberg

3. Generation of local pH gradients using nanoelectrodes to control catalysis of single H⁺-ATP synthases for nanobiotechnology
with Prof. Dr. J. Heinze (Physical Chemistry, University of Freiburg)

Teaching

• seminars 'Introduction to fluorescence spectroscopy of single-molecules'   University of Freiburg, in 1997 and in 2000

[parameters in fluorescence spectroscopy, excitation, emission, quantum yields, excitation and emission anisotropy, fluorescence lifetime, TCSPC, phosphorescence, delayed fluorescence, quenching, resonance energy transfer FRET, fluorophores, quantum dots, GFPs, single-molecule detection (SMD) in solids, spectral jumps, spectral diffusion, SMD in solution, fluorescence correlation spectroscopy FCS, etc.]

• lecture series 'single-molecule spectroscopy', 2003, University of Freiburg

 [SMD in solids, guest-host systems at low temp., in polymers, spectral jumps, spectral diffusion, light harvesting complex,
 SMD in solution, FCS, single-molecule fluorescence lifetime and anisotropy with TCSPC, fluorophores, confocal and TIRF
 microscopy, single-molecule FRET, data analysis of time trajectories, dwell times]

• 'single-molecule fluorescence resonance energy transfer I' sommer semester 2004, University of Freiburg

  [principles of FRET, fluorophores, Förster theory of FRET, calculation of quantum yields, solvent relaxtion, quenching,
  the overlap integral, determing the Förster radius, FRET efficieny and kappa squared, transition dipole moments, anisoptropy,
  limits for kappa squared, graphical solutions, distance measurements by FRET, conformational changes in ATP synthase]

• 'single-molecule fluorescence resonance energy transfer II'    winter semester 2004 / 2005, University of Freiburg

  [novel measurement methods of FRET efficiency, FRET imaging methods, immobilization methods on surfaces,
  single-molecule FRET in living cells,  data analysis for submillisecond time resolution, three-color FRET, etc.]

• occasional lectures in Physical Chemistry for undergraduate and graduate students in Chemistry, Applied Microtechnology, Biology, 1999 ff.
    [thermodynamics, kinetics, electrochemistry, quantum mechanics, spectroscopy]
   
• supervisor in the corresponding excercises to the lectures, and practical training courses (since 1991), diploma students in Chemistry and Physics, PhD students in Chemistry and Physics (since 1998 / 2002)