Picosecond time resolved fluorescence analysis is performed on different setups

from single fluorophores in cells to the nanomotor FoF1-ATP synthase

  • ps-pulsed supercontinuum laser 450-2000nm (Fianium)
  • ps-pulsed amplified laserdiode 488 nm, PicoTA 490 (Picoquant)
  • ps-pulsed laserdiode 635 nm (Picoquant)
  • solid state laser Calypso 491 nm (Cobolt)
  • frequency doubled Nd:YAG 532 nm (Coherent)
  • HeNe 594 nm (Coherent)
  • several laser diodes (405nm, 658 nm, 785 nm)
     
  • gated intensified OMA : high resolution spectra in 5 ns
  • SLM 8100 fluorescence spectrophotometer
  • ultrasensitive fast EMCCD  camera (Andor IXON 897)
    for imaging and superlocalization microscopies
  • single molecule detection with TCSPC
     (4 APDs/channels for FRET and anisotropy)
  • fluorescence correlation spectroscopy (2 channels)
  • ABEL trap
     
     
  • 2x Olympus IX 71 with xyz scanning stage (PI)
  • TIRF microscopy
  • combined with SECM
    (scanning electrochemical microscope
    with nano- and microelectrodes    )
  • Zeiss Axiovert
spectroscopy