Picosecond time resolved fluorescence analysis is performed on different setups

from single fluorophores in yeast cells to the nanomotor F_oF₁-ATP synthase

• ps-pulsed amplified laserdiode 488 nm, PicoTA 490 (Picoquant)
• ps-pulsed laserdiode 635 nm (Picoquant)
• frequency doubled Nd:YAG 532 nm (Coherent)
• argon ion laser (Spectra Physics)
• and more in the 3rd Institute of Physics (Stuttgart)

• single molecule detection with TCSPC
   (4 APDs/channels for FRET and anisotropy)
• fluorescence correlation spectroscopy (2 channels)

• gated intensified OMA : high resolution spectra in 5 ns
• sensitive CCD for imaging

• Olympus IX 71 with xyz scanning stage (PI)
• combined with SECM
  (scanning electrochemical microscope
  with nano- and microelectrodes)
• Zeiss Axiovert