Picosecond time resolved fluorescence analysis is performed on different setups

from single fluorophores in yeast cells to the nanomotor FoF1-ATP synthase

  • ps-pulsed amplified laserdiode 488 nm, PicoTA 490 (Picoquant)
  • ps-pulsed laserdiode 635 nm (Picoquant)
  • frequency doubled Nd:YAG 532 nm (Coherent)
  • argon ion laser (Spectra Physics)
  • and more in the 3rd Institute of Physics (Stuttgart)
  • single molecule detection with TCSPC
     (4 APDs/channels for FRET and anisotropy)
  • fluorescence correlation spectroscopy (2 channels)
  • gated intensified OMA : high resolution spectra in 5 ns
  • sensitive CCD for imaging
  • Olympus IX 71 with xyz scanning stage (PI)
  • combined with SECM
    (scanning electrochemical microscope
    with nano- and microelectrodes
    )
  • Zeiss Axiovert
spectroscopy